![]() In the same way, the square distribution scheme is adopted, which means that one W spherical area can be chain-replaced with the surrounding 4 ABC complex spherical areas, and one ABC area can be chain-replaced with the surrounding 4 W areas. The number of W chains that each ABC complex may be exposed to is calculated as Since each sphere includes four chains, the number of ABC compound chains that each W may If the equilateral triangle distribution scheme is adopted, it means that one W sphere area can be chain-replaced with the surrounding 3 ABC complex sphere areas, and one ABC area can be chain-replaced with the surrounding 6 W areas. Figure 8 (Sphere) Distribution of equilateral triangle and square ![]() The purple spherical surface represents the W-β end envelope surface, and the blue spherical surface represents the C-Toehold2 end envelope surface. According to the envelope surface model, W-β can replace C-toehold1 of multiple ABC complexes in the same area, such as 3 or 4. In a micro unit, if the spheres do not intersect, the two complexes will not compete for displacement after the tangent to the intersection, the β end of the W chain can enter the motion space of the A-B-C complex, and strand displacement will occur.įigure 8: Since W can react with multiple ABC complexes, the number of W chains should be as small as possible in the same reaction zone to ensure that the amount of L chains is as small as possible when the content of T chains is unknown. The length of the generatrix of the cone envelope surface is fixed, but the cone apex angle is variable, and the circle cluster composed of the edge line envelopes into an approximate hemisphere. The interface of the micro-unit is very small, approximately flat. This quadrangular pyramid can rotate around the avidin molecule and the normal direction of the sphere to produce a cone-shaped envelope surface, which is essentially the coincident surface of the respective envelope surfaces of the four chains. The four chains on the sphere approximately form a quadrangular pyramid. ![]() The spatial extension of avidin and biotin is much smaller than the length of the nucleic acid chain, which can be approximated as a point attached to the spherical surface with no actual height. Several nearest avidin molecules are combined on the spherical surface to form a spherical micro-unit. Figure 7 Enveloping cone and hemisphere in a micro unitįigure 7: A very large number of avidin molecules can be loaded on the same magnetic bead, and they are evenly distributed on the spherical surface.
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